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PeproTech poly-ig control

IL-10 modulates neutrophilic inflammation following S. pneumoniae infection. Young adult Balb/c mice (6–7 wk of age, n = 5 per group) were intranasally challenged with 5 × 10 6 cfu S. pneumoniae strain D39 (serotype 2), treated with nonspecific <t>poly-IgG</t> <t>control</t> antibody or <t>anti-IL-10</t> neutralizing antibody, and euthanized 24 h later. Total cells were recovered from BALF ( A ) and lung tissue ( B ). Differential cell counts were performed on BALF cytospin preparations or flow cytometry analysis was performed on whole lung cell homogenates. Analysis determined total neutrophil ( C and D ) and macrophage numbers ( E and F ) in BALF and lung tissue, respectively. Neutrophil numbers ( G ) and IL-1β levels ( H ) were determined at 2, 4, and 8 h after anti-IL-10 or poly-IgG control antibody treatment in the absence of infection. Statistical analysis was performed by using a Student's t -test when comparing 2 groups or 2-way ANOVA to compare all groups (* P < 0.05).

Poly Ig Control, supplied by PeproTech, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/poly-ig+control/pmc04360060-45-16-18?v=PeproTech
Average 90 stars, based on 1 article reviews

poly-ig control - by Bioz Stars, 2026-07

90/100 stars

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1) Product Images from "Enhanced inflammation in aged mice following infection with Streptococcus pneumoniae is associated with decreased IL-10 and augmented chemokine production"

Article Title: Enhanced inflammation in aged mice following infection with Streptococcus pneumoniae is associated with decreased IL-10 and augmented chemokine production

Journal: American Journal of Physiology - Lung Cellular and Molecular Physiology

doi: 10.1152/ajplung.00141.2014

Figure Legend Snippet: IL-10 modulates neutrophilic inflammation following S. pneumoniae infection. Young adult Balb/c mice (6–7 wk of age, n = 5 per group) were intranasally challenged with 5 × 10 6 cfu S. pneumoniae strain D39 (serotype 2), treated with nonspecific poly-IgG control antibody or anti-IL-10 neutralizing antibody, and euthanized 24 h later. Total cells were recovered from BALF ( A ) and lung tissue ( B ). Differential cell counts were performed on BALF cytospin preparations or flow cytometry analysis was performed on whole lung cell homogenates. Analysis determined total neutrophil ( C and D ) and macrophage numbers ( E and F ) in BALF and lung tissue, respectively. Neutrophil numbers ( G ) and IL-1β levels ( H ) were determined at 2, 4, and 8 h after anti-IL-10 or poly-IgG control antibody treatment in the absence of infection. Statistical analysis was performed by using a Student's t -test when comparing 2 groups or 2-way ANOVA to compare all groups (* P < 0.05).

Techniques Used: Infection, Control, Flow Cytometry

IL-10 has no effect on bacterial clearance. Young adult Balb/c mice (6–7 wk of age, n = 5 per group) were intranasally challenged with 5 × 10 6 cfu S. pneumoniae strain D39 (serotype 2), treated with nonspecific poly-IgG control antibody or anti-IL-10 neutralizing antibody, and euthanized 4, 24 or 48 h later. Bacteria recovered from BALF ( A ) and whole lung homogenates ( B ) were cultured on Columbia agar containing 5% defibrinated horse serum. Cultures were incubated at 37°C for 18 h. The cfu were counted and numbers were transformed into log10 values. Differential cell counts were performed on BALF cytospin preparations and neutrophil ( C ) and macrophage ( D ) total cell counts calculated. Statistical analysis was performed by using a 2-way ANOVA comparing all groups (*** P < 0.001; ** P < 0.005).

Figure Legend Snippet: IL-10 has no effect on bacterial clearance. Young adult Balb/c mice (6–7 wk of age, n = 5 per group) were intranasally challenged with 5 × 10 6 cfu S. pneumoniae strain D39 (serotype 2), treated with nonspecific poly-IgG control antibody or anti-IL-10 neutralizing antibody, and euthanized 4, 24 or 48 h later. Bacteria recovered from BALF ( A ) and whole lung homogenates ( B ) were cultured on Columbia agar containing 5% defibrinated horse serum. Cultures were incubated at 37°C for 18 h. The cfu were counted and numbers were transformed into log10 values. Differential cell counts were performed on BALF cytospin preparations and neutrophil ( C ) and macrophage ( D ) total cell counts calculated. Statistical analysis was performed by using a 2-way ANOVA comparing all groups (*** P < 0.001; ** P < 0.005).

Techniques Used: Control, Bacteria, Cell Culture, Incubation, Transformation Assay

IL-10 does not affect leak, TNF, or IL-1β levels. Young adult Balb/c mice (6–7 wk of age, n = 5 per group) were intranasally challenged with 5 × 10 6 cfu S. pneumoniae strain D39 (serotype 2), treated with nonspecific poly-IgG control antibody or anti-IL-10 neutralizing antibody, and euthanized 24 h later. BALF was recovered and serum albumin ( left ) was measured by ELISA. Lung tissue was removed postmortem and homogenized, the cell pellet was centrifuged, and the supernatant was removed. The levels of IL-1β ( middle ) and TNF ( right ) in lung homogenates were measured by ELISA. Statistical analysis was performed by using a Student's t -test (* P < 0.05).

Figure Legend Snippet: IL-10 does not affect leak, TNF, or IL-1β levels. Young adult Balb/c mice (6–7 wk of age, n = 5 per group) were intranasally challenged with 5 × 10 6 cfu S. pneumoniae strain D39 (serotype 2), treated with nonspecific poly-IgG control antibody or anti-IL-10 neutralizing antibody, and euthanized 24 h later. BALF was recovered and serum albumin ( left ) was measured by ELISA. Lung tissue was removed postmortem and homogenized, the cell pellet was centrifuged, and the supernatant was removed. The levels of IL-1β ( middle ) and TNF ( right ) in lung homogenates were measured by ELISA. Statistical analysis was performed by using a Student's t -test (* P < 0.05).

Techniques Used: Control, Enzyme-linked Immunosorbent Assay

IL-10 modulates the release of CCL3, CCL5, and CXCL10. Young adult Balb/c mice (6–7 wk of age, n = 5 per group) were intranasally challenged with 5 × 10 6 cfu S. pneumoniae strain D39 (serotype 2), treated with nonspecific poly-IgG control antibody or anti-IL-10 neutralizing antibody, and euthanized 24 h later. Lung tissue was removed postmortem and homogenized, the cell pellet was centrifuged, and the supernatant was removed. The levels of CCL2 ( A ), CCL3 ( B ), CCL4 ( C ), CCL5 ( D ), CCL11 ( E ), CCL17 ( F ), CCL21 ( G ), CCL22 ( H ), CXCL1 ( I ), CXCL9 ( J ), CXCL10 ( K ), and CXCL12 ( L ) were measured by multianalyte ELISA array. Statistical analysis was performed by using a Student's t -test (*** P < 0.0001; ** P < 0.001; * P < 0.05).

Figure Legend Snippet: IL-10 modulates the release of CCL3, CCL5, and CXCL10. Young adult Balb/c mice (6–7 wk of age, n = 5 per group) were intranasally challenged with 5 × 10 6 cfu S. pneumoniae strain D39 (serotype 2), treated with nonspecific poly-IgG control antibody or anti-IL-10 neutralizing antibody, and euthanized 24 h later. Lung tissue was removed postmortem and homogenized, the cell pellet was centrifuged, and the supernatant was removed. The levels of CCL2 ( A ), CCL3 ( B ), CCL4 ( C ), CCL5 ( D ), CCL11 ( E ), CCL17 ( F ), CCL21 ( G ), CCL22 ( H ), CXCL1 ( I ), CXCL9 ( J ), CXCL10 ( K ), and CXCL12 ( L ) were measured by multianalyte ELISA array. Statistical analysis was performed by using a Student's t -test (*** P < 0.0001; ** P < 0.001; * P < 0.05).

Techniques Used: Control, Enzyme-linked Immunosorbent Assay

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Journal: American Journal of Physiology - Lung Cellular and Molecular Physiology

Article Title: Enhanced inflammation in aged mice following infection with Streptococcus pneumoniae is associated with decreased IL-10 and augmented chemokine production

doi: 10.1152/ajplung.00141.2014

Figure Lengend Snippet: IL-10 modulates neutrophilic inflammation following S. pneumoniae infection. Young adult Balb/c mice (6–7 wk of age, n = 5 per group) were intranasally challenged with 5 × 10 6 cfu S. pneumoniae strain D39 (serotype 2), treated with nonspecific poly-IgG control antibody or anti-IL-10 neutralizing antibody, and euthanized 24 h later. Total cells were recovered from BALF ( A ) and lung tissue ( B ). Differential cell counts were performed on BALF cytospin preparations or flow cytometry analysis was performed on whole lung cell homogenates. Analysis determined total neutrophil ( C and D ) and macrophage numbers ( E and F ) in BALF and lung tissue, respectively. Neutrophil numbers ( G ) and IL-1β levels ( H ) were determined at 2, 4, and 8 h after anti-IL-10 or poly-IgG control antibody treatment in the absence of infection. Statistical analysis was performed by using a Student's t -test when comparing 2 groups or 2-way ANOVA to compare all groups (* P < 0.05).

Article Snippet: In addition, the inoculum contained 10 μg of functional grade anti-IL-10 antibody per mouse (eBioscience) or poly-Ig control (PeproTech).

Techniques: Infection, Control, Flow Cytometry

Journal: American Journal of Physiology - Lung Cellular and Molecular Physiology

Article Title: Enhanced inflammation in aged mice following infection with Streptococcus pneumoniae is associated with decreased IL-10 and augmented chemokine production

doi: 10.1152/ajplung.00141.2014

Figure Lengend Snippet: IL-10 has no effect on bacterial clearance. Young adult Balb/c mice (6–7 wk of age, n = 5 per group) were intranasally challenged with 5 × 10 6 cfu S. pneumoniae strain D39 (serotype 2), treated with nonspecific poly-IgG control antibody or anti-IL-10 neutralizing antibody, and euthanized 4, 24 or 48 h later. Bacteria recovered from BALF ( A ) and whole lung homogenates ( B ) were cultured on Columbia agar containing 5% defibrinated horse serum. Cultures were incubated at 37°C for 18 h. The cfu were counted and numbers were transformed into log10 values. Differential cell counts were performed on BALF cytospin preparations and neutrophil ( C ) and macrophage ( D ) total cell counts calculated. Statistical analysis was performed by using a 2-way ANOVA comparing all groups (*** P < 0.001; ** P < 0.005).

Article Snippet: In addition, the inoculum contained 10 μg of functional grade anti-IL-10 antibody per mouse (eBioscience) or poly-Ig control (PeproTech).

Techniques: Control, Bacteria, Cell Culture, Incubation, Transformation Assay

Journal: American Journal of Physiology - Lung Cellular and Molecular Physiology

Article Title: Enhanced inflammation in aged mice following infection with Streptococcus pneumoniae is associated with decreased IL-10 and augmented chemokine production

doi: 10.1152/ajplung.00141.2014

Figure Lengend Snippet: IL-10 does not affect leak, TNF, or IL-1β levels. Young adult Balb/c mice (6–7 wk of age, n = 5 per group) were intranasally challenged with 5 × 10 6 cfu S. pneumoniae strain D39 (serotype 2), treated with nonspecific poly-IgG control antibody or anti-IL-10 neutralizing antibody, and euthanized 24 h later. BALF was recovered and serum albumin ( left ) was measured by ELISA. Lung tissue was removed postmortem and homogenized, the cell pellet was centrifuged, and the supernatant was removed. The levels of IL-1β ( middle ) and TNF ( right ) in lung homogenates were measured by ELISA. Statistical analysis was performed by using a Student's t -test (* P < 0.05).

Article Snippet: In addition, the inoculum contained 10 μg of functional grade anti-IL-10 antibody per mouse (eBioscience) or poly-Ig control (PeproTech).

Techniques: Control, Enzyme-linked Immunosorbent Assay

Journal: American Journal of Physiology - Lung Cellular and Molecular Physiology

Article Title: Enhanced inflammation in aged mice following infection with Streptococcus pneumoniae is associated with decreased IL-10 and augmented chemokine production

doi: 10.1152/ajplung.00141.2014

Figure Lengend Snippet: IL-10 modulates the release of CCL3, CCL5, and CXCL10. Young adult Balb/c mice (6–7 wk of age, n = 5 per group) were intranasally challenged with 5 × 10 6 cfu S. pneumoniae strain D39 (serotype 2), treated with nonspecific poly-IgG control antibody or anti-IL-10 neutralizing antibody, and euthanized 24 h later. Lung tissue was removed postmortem and homogenized, the cell pellet was centrifuged, and the supernatant was removed. The levels of CCL2 ( A ), CCL3 ( B ), CCL4 ( C ), CCL5 ( D ), CCL11 ( E ), CCL17 ( F ), CCL21 ( G ), CCL22 ( H ), CXCL1 ( I ), CXCL9 ( J ), CXCL10 ( K ), and CXCL12 ( L ) were measured by multianalyte ELISA array. Statistical analysis was performed by using a Student's t -test (*** P < 0.0001; ** P < 0.001; * P < 0.05).

Article Snippet: In addition, the inoculum contained 10 μg of functional grade anti-IL-10 antibody per mouse (eBioscience) or poly-Ig control (PeproTech).

Techniques: Control, Enzyme-linked Immunosorbent Assay

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1) Product Images from "Enhanced inflammation in aged mice following infection with Streptococcus pneumoniae is associated with decreased IL-10 and augmented chemokine production"

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